Article
Knocking down gene function with an RNA aptamer expressed as part of an intron.
Nucleic acids research - 1 Aug 2010
Wang Shengchun, Zhao Xiaoching, Suran Robert, Vogt Volker M, Lis John T, Shi Hua
Abstract excerpt
We developed a powerful expression system to produce aptamers and other types of functional RNA in yeast to examine their effects. Utilizing the intron homing process, the aptamer-coding sequences were integrated into hundreds of rRNA genes, and the aptamers were transcribed at high levels by RNA polymerase I without any additional promoter being introduced into the cell. We used this system to express an aptamer...
Topics
- Aptamers, Nucleotide
- DNA-Binding Proteins
- Gene Expression Regulation
- Gene Knockdown Techniques
- Heat Shock Transcription Factors
- Introns
- Phenotype
- RNA
- Saccharomyces cerevisiae
- Transcription Factors