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Article

Correcting errors in synthetic DNA through consensus shuffling.

Nucleic acids research - 30 Mar 2005

Binkowski Brock F, Richmond Kathryn E, Kaysen James, Sussman Michael R, Belshaw Peter J

Abstract excerpt

Although efficient methods exist to assemble synthetic oligonucleotides into genes and genomes, these suffer from the presence of 1-3 random errors/kb of DNA. Here, we introduce a new method termed consensus shuffling and demonstrate its use to significantly reduce random errors in synthetic DNA. In this method, errors are revealed as mismatches by re-hybridization of the population. The DNA is fragmented, and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to MutS DNA Mismatch-Binding Protein

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Identifiers

PMID: 15800206
DOI: 10.1093/nar/gni053
PMCID: PMC1072806

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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