Article
The role of internal packing interactions in determining the structure and stability of a protein.
Journal of molecular biology - 20 May 1991
Lim W A, Sauer R T
Abstract excerpt
Cassette mutagenesis has been used to investigate how internal packing interactions help to specify a protein's three-dimensional structure and stability. Three interacting residues in the hydrophobic core of the N-terminal domain of lambda repressor were randomized combinatorially. The randomiza...
Topics
- DNA, Bacterial
- DNA-Binding Proteins
- Drug Stability
- Escherichia coli
- Genes, Synthetic
- Hot Temperature
- Models, Molecular
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Protein Binding
- Protein Conformation
- Proteins
- Repressor Proteins
- Restriction Mapping
- Thermodynamics
- Viral Proteins
- Viral Regulatory and Accessory Proteins