Article
Structural and energetic consequences of disruptive mutations in a protein core.
Biochemistry - 5 May 1992
Lim W A, Farruggio D C, Sauer R T
Abstract excerpt
We have characterized the properties of a set of variants of the N-terminal domain of lambda repressor bearing disruptive mutations in the hydrophobic core. These mutations include some that dramatically alter the total core residue volume (by up to six methylene groups) and some that place a sin...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Circular Dichroism
- DNA-Binding Proteins
- Guanidine
- Guanidines
- Hot Temperature
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation