Article
Sequence requirements for coiled-coils: analysis with lambda repressor-GCN4 leucine zipper fusions.
Science (New York, N.Y.) - 7 Dec 1990
Hu J C, O'Shea E K, Kim P S, Sauer R T
Abstract excerpt
A genetic system was developed in Escherichia coli to study leucine zippers with the amino-terminal domain of bacteriophage lambda repressor as a reporter for dimerization. This system was used to analyze the importance of the amino acid side chains at eight positions that form the hydrophobic in...
Topics
- Amino Acid Sequence
- Bacteriophage lambda
- DNA-Binding Proteins
- Escherichia coli
- Fungal Proteins
- Genetic Variation
- Leucine Zippers
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Protein Conformation
- Protein Kinases
- Random Allocation
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae Proteins
- Transcription Factors