Article
Protein stabilization by removal of unsatisfied polar groups: computational approaches and experimental tests.
Biochemistry - 18 Jun 1996
Hendsch Z S, Jonsson T, Sauer R T, Tidor B
Abstract excerpt
The role of polar and charged side chains in partially buried protein environments has been probed in a variant of Arc repressor (MYL) in which hydrophobic interactions between Met31, Tyr36, and Leu40 replace the wild-type salt-bridge interactions between Arg31, Glu36, and Arg40. In the absence o...
Topics
- Calorimetry
- Circular Dichroism
- Computer Simulation
- DNA-Binding Proteins
- Drug Stability
- Genetic Variation
- Models, Structural
- Mutagenesis, Insertional
- Point Mutation
- Protein Conformation
- Protein Denaturation
- Protein Structure, Secondary
- Recombinant Proteins
- Repressor Proteins
- Software
- Thermodynamics
- Viral Proteins
- Viral Regulatory and Accessory Proteins