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Article

Mapping the phenotypic landscape of a transcriptional repressor using deep mutational scanning and growth-based quantitative sequencing.

Nucleic acids research - 24 Feb 2026

Jansen Zachary, Le Xuan, Wei Qiyao, Kulhanek Devon L, Alperovich Nina, Vasilyeva Olga, Gilmour Andrew R, Ross David, Thyer Ross

Abstract excerpt

CymR is a TetR-family transcriptional repressor that recognizes a well-defined operator sequence in the promoter PcymRC. The native ligand cumate and several structurally related aromatic acids bind at an allosteric site and induce a conformational change in CymR, resulting in release from the DNA operator and de-repression of the promoter. The amino acid residues that contribute to these core functions have not...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41816907
DOI: 10.1093/nar/gkag206
PMCID: PMC12980060

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