Article
Active barnase variants with completely random hydrophobic cores.
Proceedings of the National Academy of Sciences of the United States of America - 28 May 1996
Axe D D, Foster N W, Fersht A R
Abstract excerpt
The central structural feature of natural proteins is a tightly packed and highly ordered hydrophobic core. If some measure of exquisite, native-like core packing is necessary for enzymatic function, this would constitute a significant obstacle to the development of novel enzymes, either by desig...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Codon
- Escherichia coli
- Genetic Variation
- Models, Structural
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Protein Structure, Secondary
- Random Allocation
- Recombinant Proteins
- Ribonucleases