Characterization of revertants of the CHO EM9 mutant arising during DNA transfection.

doi:10.1093/carcin/12.5.805

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Article

Characterization of revertants of the CHO EM9 mutant arising during DNA transfection.

Carcinogenesis - 1 May 1991

Barrows L R, Paxton M B, Kennedy K A, Thompson L H

Abstract excerpt

We have studied reversion in DNA repair deficient EM9 cells, by selection for ethylmethanesulfonate (EMS) resistance. EM9 is a mutant CHO cell line that is hypersensitive to killing by EMS and X-rays and deficient in DNA single-strand break (SSB) repair. EM9 cells were transfected with DNA from a...

Topics

Animals
Blotting, Northern
Blotting, Southern
Carcinogens
Cell Line
Cell Survival
Cricetinae
Cricetulus
DNA
DNA Probes
DNA Repair
Gene Expression Regulation
Mutation
RNA, Messenger
Transfection

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