Article
Construction of human XRCC1 minigenes that fully correct the CHO DNA repair mutant EM9.
Nucleic acids research - 11 Sept 1992
Caldecott K W, Tucker J D, Thompson L H
Abstract excerpt
The human gene that corrects the DNA repair defect of the CHO cell mutant EM9 is designated XRCC1 and is the first human gene to be cloned that has an established role in DNA strand-break repair. In this study, either an XRCC1 cosmid genomic fragment or synthetic oligonucleotides were ligated to an incomplete XRCC1 cDNA to generate two full-length XRCC1 cDNA constructs. The ability of these minigene constructs to...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cloning, Molecular
- Cosmids
- Cricetinae
- DNA Repair
- Genes
- Humans
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides