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Article

Multiple autophosphorylation site mutations of the epidermal growth factor receptor. Analysis of kinase activity and endocytosis.

The Journal of biological chemistry - 5 May 1991

Sorkin A, Waters C, Overholser K A, Carpenter G

Abstract excerpt

We have utilized site-directed mutants to study the role of autophosphorylation of the epidermal growth factor (EGF) receptor in the regulation of receptor kinase activity and ligand-induced endocytosis. A single mutation of the major autophosphorylation site, Y1173, and a double mutation of two autophosphorylation sites, Y1173 and Y1148, did not inhibit kinase activity in vivo, using PLC gamma 1 as a specific...

Topics

Animals
Cell Line
Down-Regulation
Endocytosis
ErbB Receptors
Humans
Kinetics
Mice
Mutation
Phosphorylation
Protein-Tyrosine Kinases
Substrate Specificity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tyrosine

Source

Identifiers

PMID: 2022651

Genes

EGF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2022651-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Multiple autophosphorylation site mutations of the epidermal growth factor receptor. Analysis of kinase activity and endocytosis.PMID 2022651Read ↗

Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.Semantic proximity Read ↗

Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor.Semantic proximity Read ↗

Multiple mechanisms collectively regulate clathrin-mediated endocytosis of the epidermal growth factor receptor.Semantic proximity Read ↗

Separate endocytic pathways of kinase-defective and -active EGF receptor mutants expressed in same cells.Semantic proximity Read ↗

Endocytosis and lysosomal targeting of epidermal growth factor receptors are mediated by distinct sequences independent of the tyrosine kinase domain.Semantic proximity Read ↗

Identification of p185neu sequences required for monoclonal antibody- or ligand-mediated receptor signal attenuation.Semantic proximity Read ↗

A site of tyrosine phosphorylation in the C terminus of the epidermal growth factor receptor is required to activate phospholipase C.Semantic proximity Read ↗

A dominant negative mutation suppresses the function of normal epidermal growth factor receptors by heterodimerization.Semantic proximity Read ↗

Multisite phosphorylation of the epidermal growth factor receptor. Use of site-directed mutagenesis to examine the role of serine/threonine phosphorylation.Semantic proximity Read ↗

Epidermal growth factor and membrane trafficking. EGF receptor activation of endocytosis requires Rab5a.Semantic proximity Read ↗

Kinase activity controls the sorting of the epidermal growth factor receptor within the multivesicular body.Semantic proximity Read ↗

Stimulation of mitogenic pathways through kinase-impaired mutants of the epidermal growth factor receptor.Semantic proximity Read ↗

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