Article
Kinase activity controls the sorting of the epidermal growth factor receptor within the multivesicular body.
Cell - 18 May 1990
Felder S, Miller K, Moehren G, Ullrich A, Schlessinger J, Hopkins C R
Abstract excerpt
We compared the internalization and intracellular sorting of epidermal growth factor receptor (EGF-R) and point mutant kinase-negative EGF-R separately expressed in NIH 3T3 cells lacking endogenous receptor. Both EGF-Rs internalized rapidly, but kinase-negative receptor was surface down-regulated only with monensin or at 20 degrees C. Furthermore, EGF internalized by mutant receptor alone was, in significant...
Topics
- Animals
- Cell Line
- Endocytosis
- ErbB Receptors
- Humans
- Kinetics
- Monensin
- Mutation
- Protein Kinases
- Protein-Tyrosine Kinases
- Receptors, Transferrin
- Temperature