Article
Identification of p185neu sequences required for monoclonal antibody- or ligand-mediated receptor signal attenuation.
DNA and cell biology - 1 Dec 1997
Qian X, O'Rourke D M, Drebin J, Zhao H, Wang Q, Greene M I
Abstract excerpt
Anti-p185neu antibodies downmodulate constitutively active p185neu receptors from the cell surface, which is associated with a reduction in the transformed phenotype. We have analyzed a group of mutant p185neu forms with carboxyl (C)-terminal truncations and/or an internal deletion of amino acids 1008-1057. Receptor endocytosis and degradation were examined by flow cytometric analysis and pulse-chase assays...
Topics
- Animals
- Antibodies, Monoclonal
- Binding Sites
- Down-Regulation
- Endocytosis
- Epidermal Growth Factor
- ErbB Receptors
- Mice
- Mice, Nude
- Mutation
- Phosphorylation
- Receptor, ErbB-2
- Recombinant Proteins
- Tumor Stem Cell Assay