Article
Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
The Journal of biological chemistry - 25 Apr 1992
Sorkin A, Helin K, Waters C M, Carpenter G, Beguinot L
Abstract excerpt
The role of epidermal growth factor (EGF) receptor autophosphorylation sites in the regulation of receptor functions has been studied using cells transfected with mutant EGF receptors. Simultaneous point mutation of 4 tyrosines (Y1068, Y1086, Y1148, Y1173) to phenylalanine, as well as removal of these sites by truncation of the carboxyl-terminal 123 amino acid residues, resulted in reduced receptor...
Topics
- Animals
- Base Sequence
- Blotting, Western
- Down-Regulation
- ErbB Receptors
- Isoenzymes
- Mice
- Molecular Sequence Data
- Mutation
- Phosphorylation
- Protein-Tyrosine Kinases