Article
Allele-specific enrichment: a method for the detection of low level N-ras gene mutations in acute myeloid leukemia.
Leukemia - 1 Feb 1991
Todd A V, Ireland C M, Iland H J
Abstract excerpt
Point mutations involving codons 12, 13, and 61 of the N-ras gene are found in patients with acute myeloid leukemia (AML). We have developed a sensitive assay for the analysis of these mutations which we have called allele-specific enrichment. In this protocol the polymerase chain reaction (PCR) amplifies DNA with primers that introduce new restriction sites into the normal N-ras allele only. Digestion with the...
Topics
- Amino Acid Sequence
- Blotting, Southern
- Genes, ras
- Humans
- Leukemia, Myeloid
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction