Article
A highly sensitive assay for mutant ras genes and its application to the study of presentation and relapse genotypes in acute leukemia.
Oncogene - 1 Feb 1994
Jacobson D R, Mills N E
Abstract excerpt
Most studies of ras oncogene activation use assays for ras mutations based on the polymerase chain reaction (PCR) of DNA segments containing ras exons 1 and 2, followed by allele-specific oligonucleotide (ASO) hybridization or direct sequencing, which require that to be detectable, a mutation must be present in at least 3-25% of ras alleles. Thus, studies of tissues in which only a fraction of cells contains a...
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