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Quantitative determination of the ratio of mutated to normal ras genes in the blood of leukemia patients by allele-specific PCR.

Leukemia research - 1 Sept 1994

Horikoshi T, Lenz H J, Danenberg K, Koch O M, Bertino J R, Danenberg P V

Abstract excerpt

By combining allele-specific PCR amplification and a PCR-based quantitation approach, a method has been developed to estimate the mutated K-ras gene content in the blood of AML patients as a percentage of total K-ras. One PCR primer set was designed not to discriminate between mutant K-ras and wild-type K-ras and thus amplified the total K-ras gene. The other PCR primer set was designed to be allele-specific for...

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PMID: 7934145
DOI: 10.1016/0145-2126(94)90069-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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