Article
Quantitative determination of the ratio of mutated to normal ras genes in the blood of leukemia patients by allele-specific PCR.
Leukemia research - 1 Sept 1994
Horikoshi T, Lenz H J, Danenberg K, Koch O M, Bertino J R, Danenberg P V
Abstract excerpt
By combining allele-specific PCR amplification and a PCR-based quantitation approach, a method has been developed to estimate the mutated K-ras gene content in the blood of AML patients as a percentage of total K-ras. One PCR primer set was designed not to discriminate between mutant K-ras and wi...
Topics
- Alleles
- Base Sequence
- Codon
- DNA
- DNA Primers
- DNA, Neoplasm
- Genes, ras
- Genetic Carrier Screening
- Humans
- Leukemia, Myeloid, Acute
- Molecular Sequence Data
- Monocytes
- Neoplasms, Second Primary
- Point Mutation
- Polymerase Chain Reaction
- Reference Values