Rapid and sensitive nonradioactive detection of mutant K-ras genes via 'enriched' PCR amplification.

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Article

Rapid and sensitive nonradioactive detection of mutant K-ras genes via 'enriched' PCR amplification.

Oncogene - 1 Jun 1991

Kahn S M, Jiang W, Culbertson T A, Weinstein I B, Williams G M, Tomita N, Ronai Z

Abstract excerpt

We have developed a rapid and highly sensitive nonradioactive method for the detection of a mutant codon 12 human c-K-ras allele in the presence of as many as 10(4) copies of the wild type codon 12 allele. This sensitivity is achieved by selective polymerase chain reaction (PCR) amplification of...

Topics

Adenocarcinoma
Alleles
Base Sequence
Cell Line
Colonic Neoplasms
Gene Amplification
Genes, ras
Humans
Keratinocytes
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

Source

Open in PubMed

Identifiers

PMID: 1676837

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1676837-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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