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Article

Microarray-based resequencing by apyrase-mediated allele-specific extension.

Electrophoresis - 1 Oct 2003

Ericsson Olle, Sivertsson Asa, Lundeberg Joakim, Ahmadian Afshin

Abstract excerpt

We have developed an array-based resequencing method to determine genetic alterations in putative cancer genes. The method relies on that the specificity of DNA polymerase in allele-specific extensions can be enhanced by terminating the extension reactions with apyrase and that a tiling set of primers are synthesized covering the investigated gene sequence. We report on such apyrase-mediated allele-specific...

Topics

Alleles
Apyrase
DNA Mutational Analysis
DNA Primers
Genes, ras
Humans
Melanoma
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Proto-Oncogene Mas
Proto-Oncogenes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Analysis, DNA

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Identifiers

PMID: 14595679
DOI: 10.1002/elps.200305583

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