Article
Engineering of Bacillus lipase by directed evolution for enhanced thermal stability: effect of isoleucine to threonine mutation at protein surface.
Molecular biology reports - 1 Jun 2011
Khurana Jyoti, Singh Ranvir, Kaur Jagdeep
Abstract excerpt
A lip gene from a Bacillus isolate was cloned and expressed in E. coli. By thermal denaturation analysis, T(1/2) of lipase was observed to be 7 min at 50°C with less than 10% activity after 1 h incubation at 50°C. To expand the functionality of cloned lipase, attempts have been made to create the...
Topics
- Amino Acid Substitution
- Animals
- Bacillus
- Directed Molecular Evolution
- Enzyme Stability
- Isoleucine
- Lipase
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Substrate Specificity