Improved activity and thermostability of Bacillus pumilus lipase by directed evolution.

doi:10.1016/j.jbiotec.2012.12.016

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Article

Improved activity and thermostability of Bacillus pumilus lipase by directed evolution.

Journal of biotechnology - 10 Mar 2013

Akbulut Nagihan, Tuzlakoğlu Öztürk Merve, Pijning Tjaard, İşsever Öztürk Saliha, Gümüşel Füsun

Abstract excerpt

To improve enzymatic activity of Bacillus pumilus lipases, DNA shuffling was applied to two lipase genes from local B. pumilus isolates. Using a high-throughput activity assay, the mutant with highest activity was selected. This chimeric mutant (L3-3), carrying two crossover positions and three p...

Topics

Bacillus
DNA Shuffling
Directed Molecular Evolution
Enzyme Stability
Escherichia coli
Hot Temperature
Lipase
Models, Molecular
Mutation
Recombinant Proteins
Structural Homology, Protein

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