Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants.

The Journal of biological chemistry - 9 Apr 2010

Li Kai, Bethea Heather N, Liu Jian

Abstract excerpt

Heparan sulfate (HS) is involved in essential physiological and pathophysiological functions. HS is a highly sulfated polysaccharide consisting of glucuronic acid (or iduronic acid) linked to glucosamine carrying various sulfo groups. Biosynthesis of HS involves sulfotransferases and an epimerase. The HS C(5)-epimerase converts glucuronic acid to iduronic acid. The method for determining the activity has been...

Topics

Carbohydrate Epimerases
Catalysis
Catalytic Domain
Disaccharides
Dose-Response Relationship, Drug
Glucuronic Acid
Humans
Iduronic Acid
Models, Chemical
Mutagenesis
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polysaccharides

Protein Engineering

Sulfotransferases

Tyrosine

Source

Open in PubMed Open full text

Identifiers

PMID: 20118238
DOI: 10.1074/jbc.M109.081059
PMCID: PMC2856987

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants.PMID 20118238Read ↗

Analysis of Drosophila glucuronyl C5-epimerase: implications for developmental roles of heparan sulfate sulfation compensation and 2-O-sulfated glucuronic acid.Semantic proximity Read ↗

Characterization and expression of highly active recombinant human glucuronyl C5-epimerase in mammalian cells.Semantic proximity Read ↗

Enzyme overexpression - an exercise toward understanding regulation of heparan sulfate biosynthesis.Semantic proximity Read ↗

Downstream Products are Potent Inhibitors of the Heparan Sulfate 2-O-Sulfotransferase.Semantic proximity Read ↗

Genetic analysis of the heparan modification network in Caenorhabditis elegans.Semantic proximity Read ↗

Sulfation of Glycosaminoglycans and Its Implications in Human Health and Disorders.Semantic proximity Read ↗

Targeted disruption of a murine glucuronyl C5-epimerase gene results in heparan sulfate lacking L-iduronic acid and in neonatal lethality.Semantic proximity Read ↗

Heparan sulfate proteoglycans.Semantic proximity Read ↗

Heparan sulfate synthesized by mouse embryonic stem cells deficient in NDST1 and NDST2 is 6-O-sulfated but contains no N-sulfate groups.Semantic proximity Read ↗

Streamlining Sulfated Oligosaccharide and Glycan Synthesis with Engineered Mutant 6-SulfoGlcNAcases.Semantic proximity Read ↗

QSulf1 remodels the 6-O sulfation states of cell surface heparan sulfate proteoglycans to promote Wnt signaling.Semantic proximity Read ↗

Galectin-3-binding protein inhibits extracellular heparan 6- <i>O</i> -endosulfatse Sulf-2Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.