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Article

Evolutionarily conserved and functionally important residues in the I-CeuI homing endonuclease.

Nucleic acids research - 1 Jul 1997

Turmel M, Otis C, Côté V, Lemieux C

Abstract excerpt

Two approaches were used to discern critical amino acid residues for the function of the I- Ceu I homing endonuclease: sequence comparison of subfamilies of homologous proteins and genetic selection. The first approach revealed residues potentially involved in catalysis and DNA recognition. Because I- Ceu I is lethal in Escherichia coli , enzyme variants not perturbing cell viability were readily selected from an...

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Identifiers

PMID: 9185572
DOI: 10.1093/nar/25.13.2610
PMCID: PMC146779

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