Article
Cell cycle-dependent strand bias for UV-induced mutations in the transcribed strand of excision repair-proficient human fibroblasts but not in repair-deficient cells.
Molecular and cellular biology - 1 Apr 1991
McGregor W G, Chen R H, Lukash L, Maher V M, McCormick J J
Abstract excerpt
To study the effect of nucleotide excision repair on the spectrum of mutations induced in diploid human fibroblasts by UV light (wavelength, 254 nm), we synchronized repair-proficient cells and irradiated them when the HPRT gene was about to be replicated (early S phase) so that there would be no time for repair in that gene before replication, or in G1 phase 6 h prior to S, and determined the kinds and location...
Topics
- Base Sequence
- Cell Line
- DNA Damage
- DNA Repair
- Fibroblasts
- G1 Phase
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Interphase
- Kinetics
- Molecular Sequence Data