Article
Mutagenicity and mutational spectrum of N-methyl-N'-nitro-N-nitrosoguanidine in the hprt gene in G1-S and late S phase of diploid human fibroblasts.
Cancer research - 15 Jun 1993
Yang J L, Lin J G, Hu M C, Wu C W
Abstract excerpt
To investigate the effect of DNA replication on the mutation spectrum induced in diploid human fibroblasts by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), cells were synchronized and exposed to MNNG either at the G1-S border or in late S phase, and the mutations in the hypoxanthine (guanine) phos...
Topics
- Amino Acid Sequence
- Aphidicolin
- Base Sequence
- Cell Cycle
- DNA
- DNA Replication
- Diploidy
- Fibroblasts
- G1 Phase
- Guanine
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Methylnitronitrosoguanidine
- Methyltransferases
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Polymerase Chain Reaction