Article
Repertoire shift in the humoral response to phosphocholine-keyhole limpet hemocyanin: VH somatic mutation in germinal center B cells impairs T15 Ig function.
Journal of immunology (Baltimore, Md. : 1950) - 15 May 2003
Wiens Gregory D, Brown McKay, Rittenberg Marvin B
Abstract excerpt
Phosphocholine (PC) is a naturally occurring Ag common to many pathogenic microorganisms. Early in the primary response to PC conjugated to keyhole limpet hemocyanin (KLH), T15 Id(+) Abs constitute >90% of the serum Ig in BALB/c mice. During the late primary and memory response to PC-protein, a shift in the repertoire occurs and T15 Id(+) Abs lose dominance. In this study, we use immunohistochemistry and single...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Anti-Idiotypic
- B-Lymphocyte Subsets
- Base Sequence
- Binding Sites, Antibody
- Cells, Cultured
- Female
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Germinal Center
- Hemocyanins
- Immunization
- Immunoglobulin Idiotypes