Article
Generation of stable monoclonal antibody-producing B cell receptor-positive human memory B cells by genetic programming.
Nature medicine - 1 Jan 2010
Kwakkenbos Mark J, Diehl Sean A, Yasuda Etsuko, Bakker Arjen Q, van Geelen Caroline M M, Lukens Michaël V, van Bleek Grada M, Widjojoatmodjo Myra N, Bogers Willy M J M, Mei Henrik, Radbruch Andreas, Scheeren Ferenc A, Spits Hergen, Beaumont Tim
Abstract excerpt
The B cell lymphoma-6 (Bcl-6) and Bcl-xL proteins are expressed in germinal center B cells and enable them to endure the proliferative and mutagenic environment of the germinal center. By introducing these genes into peripheral blood memory B cells and culturing these cells with two factors produced by follicular helper T cells, CD40 ligand (CD40L) and interleukin-21 (IL-21), we convert them to highly...
Topics
- Animals
- Antibodies, Monoclonal
- Antibodies, Neutralizing
- Antibodies, Viral
- B-Lymphocyte Subsets
- Cell Line
- Cytidine Deaminase
- DNA-Binding Proteins
- Enzyme Activation