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Article

Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation.

Nucleic acids research - 15 Nov 2002

Lee Yin-Fai, Tawfik Dan S, Griffiths Andrew D

Abstract excerpt

In vitro compartmentalisation (IVC), a technique for selecting genes encoding enzymes based on compartmentalising gene translation and enzymatic reactions in emulsions, was used to investigate the interaction of the DNA cytosine-5 methyltransferase M.HhaI with its target DNA (5'-GCGC-3'). Crystallography shows that the active site loop from the large domain of M.HhaI interacts with a flipped-out cytosine (the...

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Identifiers

PMID: 12433997
DOI: 10.1093/nar/gkf617
PMCID: PMC137165

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