Article
Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation.
Nucleic acids research - 15 Nov 2002
Lee Yin-Fai, Tawfik Dan S, Griffiths Andrew D
Abstract excerpt
In vitro compartmentalisation (IVC), a technique for selecting genes encoding enzymes based on compartmentalising gene translation and enzymatic reactions in emulsions, was used to investigate the interaction of the DNA cytosine-5 methyltransferase M.HhaI with its target DNA (5'-GCGC-3'). Crystallography shows that the active site loop from the large domain of M.HhaI interacts with a flipped-out cytosine (the...
Topics
- Amino Acids
- Base Sequence
- Binding Sites
- Codon
- DNA
- DNA-Cytosine Methylases
- Emulsions
- Gene Library
- Models, Genetic
- Models, Molecular
- Mutation
- Protein Biosynthesis