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Article

Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization.

Protein engineering, design & selection : PEDS - 1 Jan 2004

Cohen Helen M, Tawfik Dan S, Griffiths Andrew D

Abstract excerpt

Engineering the specificity of DNA-modifying enzymes has proven extremely challenging, as sequence recognition by these enzymes is poorly understood. Here we used directed evolution to generate a variant of HaeIII methyltransferase that efficiently methylates a novel target site. M.HaeIII methylates the internal cytosine of the canonical sequence GGCC, but there is promiscuous methylation of a variety of...

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Identifiers

PMID: 14985532
DOI: 10.1093/protein/gzh001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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