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Article

Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases.

PloS one - 6 Dec 2010

Stapleton James A, Swartz James R

Abstract excerpt

BACKGROUND: [FeFe] hydrogenase enzymes catalyze the formation and dissociation of molecular hydrogen with the help of a complex prosthetic group composed of common elements. The development of energy conversion technologies based on these renewable catalysts has been hindered by their extreme oxygen sensitivity. Attempts to improve the enzymes by directed evolution have failed for want of a screening platform...

Topics

Catalysis
Cell-Free System
DNA
Dimethylpolysiloxanes
Directed Molecular Evolution
Escherichia coli
Hydrogen
Hydrogenase
In Vitro Techniques
Iron
Microfluidics
Models, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Oxygen

Polymerase Chain Reaction

Spectrometry, Fluorescence

Source

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Identifiers

PMID: 21151915
DOI: 10.1371/journal.pone.0015275
PMCID: PMC2997796

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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