Article
Mutational specificities of environmental carcinogens in the lacI gene of Escherichia coli: IV. The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.
Carcinogenesis - 1 Feb 1991
Jiao J L, Zielenska M, Anderson M W, Glickman B W
Abstract excerpt
We have determined the mutational specificity of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) by the characterization of 58 induced Escherichia coli lacI-d mutants at the DNA sequence level. Metabolic activation of NNK was carried out using the S9 fraction from Aroclor 1254-treated rats. G:C----A:T transitions dominated the spectrum, accounting for 55% of the mutations...
Topics
- Bacterial Proteins
- Base Sequence
- Carcinogens
- Environmental Exposure
- Escherichia coli
- Escherichia coli Proteins
- Lac Repressors
- Molecular Sequence Data
- Mutation
- Nitrosamines