Article
Prp43 bound at different sites on the pre-rRNA performs distinct functions in ribosome synthesis.
Molecular cell - 25 Nov 2009
Bohnsack Markus T, Martin Roman, Granneman Sander, Ruprecht Maike, Schleiff Enrico, Tollervey David
Abstract excerpt
Yeast ribosome synthesis requires 19 different RNA helicases, but none of their pre-rRNA-binding sites were previously known, making their precise functions difficult to determine. Here we identify multiple binding sites for the helicase Prp43 in the 18S and 25S rRNA regions of pre-rRNAs, using UV crosslinking. Binding in 18S was predominantly within helix 44, close to the site of 18S 3' cleavage, in which Prp43...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cross-Linking Reagents
- DEAD-box RNA Helicases
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- RNA Precursors
- RNA, Fungal
- RNA, Ribosomal