Article
Dynamics of the putative RNA helicase Spb4 during ribosome assembly in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Oct 2011
García-Gómez Juan José, Lebaron Simon, Froment Carine, Monsarrat Bernard, Henry Yves, de la Cruz Jesús
Abstract excerpt
Spb4 is a putative ATP-dependent RNA helicase that is required for proper processing of 27SB pre-rRNAs and therefore for 60S ribosomal subunit biogenesis. To define the timing of association of this protein with preribosomal particles, we have studied the composition of complexes that copurify with Spb4 tagged by tandem affinity purification (TAP-tagged Spb4). These complexes contain mainly the 27SB pre-rRNAs and...
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