Article
Two prostate carcinoma cell lines demonstrate abnormalities in tumor suppressor genes.
Journal of surgical oncology - 1 Jan 1991
Rubin S J, Hallahan D E, Ashman C R, Brachman D G, Beckett M A, Virudachalam S, Yandell D W, Weichselbaum R R
Abstract excerpt
Two prostate carcinoma cell lines, DU-145 and PC-3, were examined for abnormalities in the retinoblastoma (Rb) and the p53 putative tumor suppressor genes. We found an abnormal Rb gene product in DU-145 using Western blot analysis. Polymerase chain reaction amplification followed by direct DNA sequencing demonstrated a base substitution mutation that generates a stop codon in exon 21. On Northern, Southern, and...
Topics
- Base Sequence
- Chromatography, Paper
- DNA, Neoplasm
- Genes, Retinoblastoma
- Genes, Tumor Suppressor
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Prostatic Neoplasms