Sequential emergence of distinct resistance phenotypes in murine erythroleukemia cells under adriamycin selection: decreased anthracycline uptake precedes increased P-glycoprotein expression.

Abstract excerpt

First-step Adriamycin (doxorubicin)-resistant mutants of the murine erythroleukemia cell line PC4 were cloned from Adriamycin-containing (10 ng/ml) methylcellulose at a frequency of 3 x 10(-4). They demonstrated 1.6- to 2.4-fold stable resistance to Adriamycin. Most were cross-resistant to etoposide, but not to vincristine, and were without enhanced expression of mdr genes, which code for P-glycoproteins. Two...

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Animals
• Biological Transport
• Cell Line
• Cell Survival
• Daunorubicin
• Doxorubicin
• Drug Resistance
• Etoposide
• Kinetics
• Leukemia, Experimental