Article
Direct selection for ribozyme cleavage activity in cells.
RNA (New York, N.Y.) - 1 Nov 2009
Chen Xi, Denison Lisa, Levy Matthew, Ellington Andrew D
Abstract excerpt
Selection may prove to be a powerful tool for the generation of functional RNAs for in vivo genetic regulation. However, traditional in vitro selection schemes do not mimic physiological conditions, and in vivo selection schemes frequently use small pool sizes. Here we describe a hybrid in vitro/in vivo selection scheme that overcomes both of these disadvantages. In this new method, PCR-amplified expression...
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