Article
Evaluating and enhancing ribozyme reaction efficiency in mammalian cells.
Nature biotechnology - 1 Sept 1997
Jones J T, Sullenger B A
Abstract excerpt
The ability of ribozymes to cleave specific transcripts and repair defective RNAs in the test tube has engendered speculation about their potential clinical utility. Therapeutic development has been hindered by an inability to evaluate and optimize the efficiency of RNA catalysis in vivo. We desc...
Topics
- Animals
- Cells, Cultured
- DNA Repair
- Genetic Therapy
- Mammals
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- RNA
- RNA, Catalytic
- Transfection