Article
Development of a selection assay for small guide RNAs that drive efficient site-directed RNA editing.
Nucleic acids research - 24 Apr 2023
Diaz Quiroz Juan Felipe, Ojha Namrata, Shayhidin Elnur E, De Silva Dasuni, Dabney Jesse, Lancaster Amy, Coull James, Milstein Stuart, Fraley Andrew W, Brown Christopher R, Rosenthal Joshua J C
Abstract excerpt
A major challenge confronting the clinical application of site-directed RNA editing (SDRE) is the design of small guide RNAs (gRNAs) that can drive efficient editing. Although many gRNA designs have effectively recruited endogenous Adenosine Deaminases that Act on RNA (ADARs), most of them exceed the size of currently FDA-approved antisense oligos. We developed an unbiased in vitro selection assay to identify...
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