Article
Spatiotemporal regulation of early lipolytic signaling in adipocytes.
The Journal of biological chemistry - 13 Nov 2009
Martin Sally, Okano Satomi, Kistler Carol, Fernandez-Rojo Manuel A, Hill Michelle M, Parton Robert G
Abstract excerpt
Hormone-sensitive lipase (HSL) is a key enzyme regulating the acute activation of lipolysis. HSL functionality is controlled by multiple phosphorylation events, which regulate its association with the surface of lipid droplets (LDs). We determined the progression and stability of HSL phosphorylation on individual serine residues both spatially and temporally in adipocytes using phospho-specific antibodies. Within...
Topics
- 1-Methyl-3-isobutylxanthine
- 3T3-L1 Cells
- Adipocytes
- Adrenergic beta-3 Receptor Agonists
- Adrenergic beta-Agonists
- Animals
- Blotting, Western
- Cells, Cultured
- Dioxoles
- Fluorescent Antibody Technique
- Immunoenzyme Techniques
- Isoproterenol
- Lipolysis