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Article

Purification and mechanism of human aldehyde oxidase expressed in Escherichia coli.

Drug metabolism and disposition: the biological fate of chemicals - 1 Dec 2009

Alfaro Joshua F, Joswig-Jones Carolyn A, Ouyang Wenyun, Nichols Joseph, Crouch Gregory J, Jones Jeffrey P

Abstract excerpt

Human aldehyde oxidase 1 (AOX1) has been subcloned into a vector suitable for expression in Escherichia coli, and the protein has been expressed. The resulting protein is active, with sulfur being incorporated in the molybdopterin cofactor. Expression levels are modest, but 1 liter of cells supplies enough protein for both biochemical and kinetic characterization. Partial purification is achieved by nickel...

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Identifiers

PMID: 19741035
DOI: 10.1124/dmd.109.029520
PMCID: PMC2784701

Genes

AOX1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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