Article
Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 8 Sept 2009
Tanrikulu I Caglar, Schmitt Emmanuelle, Mechulam Yves, Goddard William A, Tirrell David A
Abstract excerpt
Incorporation of noncanonical amino acids into cellular proteins often requires engineering new aminoacyl-tRNA synthetase activity into the cell. A screening strategy that relies on cell-surface display of reactive amino acid side-chains was used to identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants that allow efficient incorporation of the methionine (Met) analog azidonorleucine (Anl). We...
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