Article
Identification of secreted bacterial proteins by noncanonical amino acid tagging.
Proceedings of the National Academy of Sciences of the United States of America - 7 Jan 2014
Mahdavi Alborz, Szychowski Janek, Ngo John T, Sweredoski Michael J, Graham Robert L J, Hess Sonja, Schneewind Olaf, Mazmanian Sarkis K, Tirrell David A
Abstract excerpt
Pathogenic microbes have evolved complex secretion systems to deliver virulence factors into host cells. Identification of these factors is critical for understanding the infection process. We report a powerful and versatile approach to the selective labeling and identification of secreted pathogen proteins. Selective labeling of microbial proteins is accomplished via translational incorporation of...
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