Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris.

doi:10.1021/bi802178k

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Article

Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris.

Biochemistry - 31 Mar 2009

Young Travis S, Ahmad Insha, Brock Ansgar, Schultz Peter G

Abstract excerpt

To increase the utility of protein mutagenesis with unnatural amino acids, a recombinant expression system in the methylotrophic yeast Pichia pastoris was developed. Aminoacyl-tRNA synthetase/suppressor tRNA (aaRS/tRNA(CUA)) pairs previously evolved in Saccharomyces cerevisiae to be specific for...

Topics

Amino Acyl-tRNA Synthetases
Escherichia coli Proteins
Humans
Mass Spectrometry
Phenotype
Pichia
Protein Engineering
RNA, Transfer

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