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Article

Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris.

Biochemistry - 31 Mar 2009

Young Travis S, Ahmad Insha, Brock Ansgar, Schultz Peter G

Abstract excerpt

To increase the utility of protein mutagenesis with unnatural amino acids, a recombinant expression system in the methylotrophic yeast Pichia pastoris was developed. Aminoacyl-tRNA synthetase/suppressor tRNA (aaRS/tRNA(CUA)) pairs previously evolved in Saccharomyces cerevisiae to be specific for unnatural amino acids were inserted between eukaryotic transcriptional control elements and stably incorporated into...

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PMID: 19265424
DOI: 10.1021/bi802178k

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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