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Article

Cell-selective metabolic labeling of proteins.

Nature chemical biology - 1 Oct 2009

Ngo John T, Champion Julie A, Mahdavi Alborz, Tanrikulu I Caglar, Beatty Kimberly E, Connor Rebecca E, Yoo Tae Hyeon, Dieterich Daniela C, Schuman Erin M, Tirrell David A

Abstract excerpt

Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not...

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Identifiers

PMID: 19668194
DOI: 10.1038/nchembio.200
PMCID: PMC3176724

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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