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Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant.

ACS chemical biology - 15 Feb 2013

Wang Yane-Shih, Fang Xinqiang, Chen Hsueh-Ying, Wu Bo, Wang Zhiyong U, Hilty Christian, Liu Wenshe R

Abstract excerpt

When coexpressed with its cognate amber suppressing tRNACUAPyl(CUA), a pyrrolysyltRNA synthetase mutant N346A/C348A is able to genetically incorporate 12 meta-substituted phenylalanine derivatives into proteins site-specifically at amber mutation sites in Escherichia coli. These genetically encoded noncanonical amino acids resemble phenylalanine in size and contain diverse bioorthogonal functional groups such as...

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PMID: 23138887
DOI: 10.1021/cb300512r
PMCID: PMC3574229

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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