Article
A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA.
ACS chemical biology - 18 Dec 2020
Nguyen Kim, Kubota Miles, Arco Jon Del, Feng Chao, Singha Monika, Beasley Samantha, Sakr Jasmine, Gandhi Sunil P, Blurton-Jones Matthew, Fernández Lucas Jesus, Spitale Robert C
Abstract excerpt
Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for...
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