Article
Development of a real-time PCR detection method for a FCGR2A polymorphism in the LightCycler and application in the heparin-induced thrombocytopenia syndrome.
Clinical biochemistry - 1 Nov 2009
Kroupis Christos, Theodorou Maria, Kounavi Maria, Oliveira Samantha C, Iliopoulou Eugenia, Mavri-Vavayanni Mary, Melissari Efthimia N, Degiannis Dimitrios
Abstract excerpt
OBJECTIVE: The FcgammaRIIa receptor is responsible for the activation of platelets by antibodies in heparin-induced thrombocytopenia (HIT). The c.497G>A polymorphism in the corresponding FCG2RA gene (H131R) has been implicated in the HIT syndrome and we aimed at its rapid and reliable determination. DESIGN AND METHODS: We designed a novel asymmetric real-time PCR method in the LightCycler that uses two...
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