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Article

Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler.

Clinica chimica acta; international journal of clinical chemistry - 1 Apr 2008

Kroupis Christos, Christopoulos Kostantinos, Devetzoglou Myrto, Tsiagas Ioannis, Lianidou Evi S

Abstract excerpt

BACKGROUND: 5382insC BRCA1 frameshift mutation is a common founder mutation for many populations worldwide and a high-risk allele for the development of hereditary breast and/or ovarian cancer. Our goal was to develop a novel, reliable and rapid method for its detection. METHODS: We developed an asymmetric real-time PCR method with hybridization probes in the LightCycler. Genotyping was performed by melting curve...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 18242166
DOI: 10.1016/j.cca.2007.12.024

Genes

BRCA1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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