Article
Single nucleotide variation detection by ligation of universal probes on a 3D poyacrylamide gel DNA microarray.
Human mutation - 1 Oct 2009
Tang Jing, Li Yanqiang, Pan Zhiqiang, Guo Yubai, Ma Jie, Ning Sheng, Xiao Pengfeng, Lu Zuhong
Abstract excerpt
We attempted to simultaneously analyze single nucleotide variations (SNVs) in a number of samples by integrating the high fidelity of ligation of universal probes with the robustness of three-dimensional (3D) polyacrylamide gel DNA microarray. By performing a ligation reaction between the 5' phosphate terminus of the sequencing primer and the 3' hydroxyl terminus of the labeled probe, we accurately identified a...
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