Article

Multiplex single-molecule DNA barcoding using an oligonucleotide ligation assay

2018-02-13

Abstract excerpt

Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence. We demonstrated that 7-nt long DNA barcodes have the optimal short length to ascertain specificity whi...

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Literature Corpus work: ff34a7b1-22ff-5965-9db1-a0e81a02212d
DOI: 10.1101/265215

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